This laboratory manual provides a comprehensive collection of experimental procedures for research using mosquitoes. It includes basic methods for mosquito rearing, egg collection, scoring mating status, scoring reproductive and host-seeking behaviors, visualizing transcript and protein expression, and evaluating physiological responses to stimuli. There are also protocols for genetic manipulation of mosquitoes using RNAi and CRISPR-Cas9 genome editing. Methods to evaluate insecticide resistance are also described.
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This laboratory manual provides a comprehensive collection of experimental procedures for research using mosquitoes. It includes basic methods for mosquito rearing, egg collection, scoring mating status, scoring reproductive and host-seeking behaviors, visualizing transcript and protein expression, and evaluating physiological responses to stimuli. There are also protocols for genetic manipulation of mosquitoes using RNAi and CRISPR-Cas9 genome editing. Methods to evaluate insecticide resistance are also described.
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