e1ectr0ph0re515 (PF6E) wa51nvented 6are1y 10 year5 a90, 6ut ha5 a1ready 6ec0me a maj0r techn14ue m01ecu- 610109y, 1ar w1th hundred5 0f 0ften the ca5e w1th new techn14ue5 the1r 5ta9e5, m05t pu611cat10n5 are 0- dated 6y the t1me they reach pr1nt. F0r rea50n, a num6er 0fthe auth0r5 0f 600k have effect1ve1y 5erved ph0ne c0n5u1tant5 0n PF6E app11cat10n5 f0r 5evera1 year5. 8y n0w, many 0f the maj0r meth0d5 have 6een te5ted and 1mpr0ved that we fee1 a 600k 0f techn14ue5 W0ve Dur1n9 the prepara- Meth0d5, and 7~, tw0 0ther 600k5 ...
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e1ectr0ph0re515 (PF6E) wa51nvented 6are1y 10 year5 a90, 6ut ha5 a1ready 6ec0me a maj0r techn14ue m01ecu- 610109y, 1ar w1th hundred5 0f 0ften the ca5e w1th new techn14ue5 the1r 5ta9e5, m05t pu611cat10n5 are 0- dated 6y the t1me they reach pr1nt. F0r rea50n, a num6er 0fthe auth0r5 0f 600k have effect1ve1y 5erved ph0ne c0n5u1tant5 0n PF6E app11cat10n5 f0r 5evera1 year5. 8y n0w, many 0f the maj0r meth0d5 have 6een te5ted and 1mpr0ved that we fee1 a 600k 0f techn14ue5 W0ve Dur1n9 the prepara- Meth0d5, and 7~, tw0 0ther 600k5 rev1ew1n9 PF6E were pu6115hed. H0wever, the pre5ent w0rk the f1r5t t0 1nc0rp0rate the pr0t0c01 5ty1e, the ha11mark 0f the Meth0d51n that ha5 made that appr0ach the pa5t. 1n add1t10n, many 0f the chapter51nc1ude 0n he1pfu11n 5trate91e5 p1ann1n9 exper1ment5. 7he part 0f the 600k c0nta1n5 the0r1e5 and 065ervat10n5 e1uc1dat1n9 the c0mp1ex 6ehav10r 0f DNA dur1n9 PF6E. F0r any 91ven app11cat10n 0f PF6E, the reader p- ceed 6y a a55em611n9 pr0t0c01 fr0m m0re than 0ne chapter (e. 9. , CHEF, preparat10n 0f a9ar05e 610ck5, and mapp1n9 5trate91e5). 70 he1p pr0ce55, th15 we have tr1ed hard t0 pr0v1de an 1ndex and cr055-reference5 that ea5y acce55 t0 the meth0d5. 1t may 6e u5efu1 t0 read chapter5 n0t 1mmed1ate1y re1ated t0 the cat10n m1nd. F0r examp1e, a u5er 0f CHEF may f1nd he1pfu1 h1nt51n the chapter5 0n F16E, 0FA6E, 0r 7AFE. Certa1n meth0d5, 5uch a5 the preparat10n 0f yea5t marker5, are d0ne 50mewhat d1fferent1y d1fferent 1a65, and the reader f1nd 5evera1 pr0- fr0m wh1ch t0 ch005e.
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New. Print on demand Contains: Illustrations, black & white. Methods in Molecular Biology . XIII, 481 p. 99 illus. Intended for professional and scholarly audience.
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