Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, observing multiple cellular activities, examining membrane cytoarchitecture, confocal imaging of live cell function, observation of intact organ physiology, and lifetime imaging, plus automated clinical imaging cytometry. The chapters introduce new methods by describing how they overcome limitations inherent in previous techniques. The authors address software, hardware and ...
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Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, observing multiple cellular activities, examining membrane cytoarchitecture, confocal imaging of live cell function, observation of intact organ physiology, and lifetime imaging, plus automated clinical imaging cytometry. The chapters introduce new methods by describing how they overcome limitations inherent in previous techniques. The authors address software, hardware and other equpment concerns. Additionally, this book reviews current applications and stimulates new developments in optical microscopy, encouraging novel uses and future technical advances. Features: * Includes recent comprehensive descriptions of: * Caged compounds, fluorescence ratio imaging and CCD video cameras * Simultaneous multiple detection and real-time fluorescence microscopy * Simultaneous DIC and quantitative LLF video imaging * Total internal reflectance, time resolved and automated fluorescence microscopy * Laser scanning confocal microscopy * Imaging for calcium measurement, membranes, glycoproteins, living cells and cancer cells
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