This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1910 Excerpt: ... designated xylol, benzol, etc. 51. Imbedding. It is best to use fresh paraffin for imbedding and if desired with a melting point higher than that of the infiltration paraffin, --50 C. paraffin (42 paraffin 1 part, 54 paraffin 2 parts), answers well in a room of 19 to 20 C, and will be generally used. If the cutting is ...
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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1910 Excerpt: ... designated xylol, benzol, etc. 51. Imbedding. It is best to use fresh paraffin for imbedding and if desired with a melting point higher than that of the infiltration paraffin, --50 C. paraffin (42 paraffin 1 part, 54 paraffin 2 parts), answers well in a room of 19 to 20 C, and will be generally used. If the cutting is to be done in a room of lower temperature, a softer grade of paraffin may be used for imbedding; if at a higher temperature, a harder paraffin should be chosen; 54 C. paraffin giving good results when summer work is necessary. As a general rule, hard tissues require a harder imbedding paraffin which is also better when very thin sections are desired. Large sections which usually must also be relatively thicker need a softer paraffin. It is better to work with a paraffin harder than the room temperature itself would call for and then regulate the cutting temperature by placing a source of heat such as an electric light nearer or farther away from the microtome knife. Make a small paper box, fill it with the melted imbedding paraffin; transfer to it the tissue from the paraffin oven, arrange it carefully in the box in the way you wish it for cutting, and cool the mass by floating the box on a dish of cold water. 52. In embedding in paraffin observe the following rules: (1) Take no more paraffin (no larger box) than is needed to form a mass of convenient size around the specimen. The aim is to have as homogeneous a mass as possible; paraffin tends to crystallize if it cools slowly, hence the smaller the mass the more rapidly may it be cooled. (2) Let the imbedding paraffin when poured into the box be several degrees above its melting point, and the tissue likewise should have an equal temperature. Should the imbedding paraffin (or t...
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