This book is a guide to the methodology of in situ hybridization --the sensitive new technique for studying the changes in genetic expression in specific tissue cells. Compared to known chemical methods for measurement, in situ hybridization has the advantage of greater exactness both in terms of the genetic information being expressed, and the identification of the cells in which that information is carried. This work is the first reference for investigators in the neurosciences interested in starting work in this ...
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This book is a guide to the methodology of in situ hybridization --the sensitive new technique for studying the changes in genetic expression in specific tissue cells. Compared to known chemical methods for measurement, in situ hybridization has the advantage of greater exactness both in terms of the genetic information being expressed, and the identification of the cells in which that information is carried. This work is the first reference for investigators in the neurosciences interested in starting work in this exciting new field. The contributing authors, whose diverse backgrounds include neuro-, developmental and microbiology were selected because of their groundbreaking work in developing in situ techniques in their various experimental systems. Some of the chapters are concerned with general biological approaches to methodology, while others discuss more specific applications to neurobiology. The focus in all the chapters in on methods, so that readers can develop individualized experimental protocols applying this powerful technique to their own research needs.
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