In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are ...
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In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
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2024,
Springer-Verlag New York Inc., New York, NY
ISBN-13: 9781071636893
4th edition 2024
Hardcover
2019,
Humana, New York, NY
ISBN-13: 9781493993284
3rd Softcover Reprint of the Original 3rd 2018 edition
Trade paperback
2018,
Humana, New York, NY
ISBN-13: 9781493985661
3rd 2018 edition
Hardcover
2016,
Humana, Totowa, NJ
ISBN-13: 9781493961115
2nd Softcover Reprint of the Original 2nd 2012 edition
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2011,
Humana, Totowa, NJ
ISBN-13: 9781617793240
2nd 2012 edition
Hardcover
2010,
Humana Press Inc., Totowa, NJ
ISBN-13: 9781617375880
Paperback
2005,
Humana Press
ISBN-13: 9781588294692
Hardcover
All Editions of Handbook of Elispot: Methods and Protocols
Books by Alexander E Kalyuzhny