Contributions to Embryology, No. 47: On the Differential Reaction to Vital Dyes Exhibited by the Two Great Groups of Connective-Tissue Cells (Classic Reprint)
Contributions to Embryology, No. 47: On the Differential Reaction to Vital Dyes Exhibited by the Two Great Groups of Connective-Tissue Cells (Classic Reprint)
Excerpt from Contributions to Embryology, No. 47: On the Differential Reaction to Vital Dyes Exhibited by the Two Great Groups of Connective-Tissue Cells Recent studies with entirely new methods, for example, the beautiful cyto logical analyses Of tissue cultures by M. R. And W. H. Lewis, and similar in vivo studies on Amphibia by E. L. And E. R. Clark, all support this simple idea. 'the application of coloring-matters to the living animal - the so-called intravitam staining - has furnished us with a unique method for the ...
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Excerpt from Contributions to Embryology, No. 47: On the Differential Reaction to Vital Dyes Exhibited by the Two Great Groups of Connective-Tissue Cells Recent studies with entirely new methods, for example, the beautiful cyto logical analyses Of tissue cultures by M. R. And W. H. Lewis, and similar in vivo studies on Amphibia by E. L. And E. R. Clark, all support this simple idea. 'the application of coloring-matters to the living animal - the so-called intravitam staining - has furnished us with a unique method for the recognition not merely of fine structural but also of physiological cell differences. Very striking results have been obtained with them, especially as regards the connective-tissue cells, in the case of animals which have been submitted to treatment with trypan blue and with isamine blue'. These dyes affect in a sharply different manner the two great cell groups Of the connective tissue and enable one to segregate them, with far greater precision than has hitherto been possible, into two definite cell classe???. It is unnecessary for us to maintain, however, that this striking reaction can serve as an utterly reliable criterion of cell species and of cell relationships or transforma tions. 'it is necessary only that we recognize in it a selective histo-pathological reagent of great beauty which separates sharply the strains of connective-tissue cells existing at any one time into two functional types without appreciable inter grades'i' From the standpoint of the physiological significance of cell differentia tion, this fact justifies all the scrutiny we can accord it, but especially as it involves the whole question of the fundamental nature of the reaction between living proto plasm and these dyes. We have attempted, consequently, in the present memoir, to describe with appropriate detail the biological peculiarities of the two connective tissue cell types as far as they may be disclosed by their differential behavior towards such substances as are embraced in the numerous dyestuffs of the acid-azo class. It is a safe prediction that he who has practised the study of living cells will not willingly revert to other methods, except for their admitted value as controls.' It is a curious commentary on the history of histological research that the micro tome has greatly discouraged methods of direct observation of the living or sur viving cell, methods which may now bring to their aid modern improvements in optical equipment and the discovery of specific coloring methods with vital dyes. He who has prosecuted with patience such studies upon the connective tissue has forced upon him the conviction of its unique effectiveness in differentiating cell types where the fixed picture leads to confusion. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at ... This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
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