This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1887 edition. Excerpt: ...the reagents are applied by a pipette at one edge of the cover-glass, and withdrawn from beneath the cover by means of a strip of filter paper placed at the opposite edge. Cover-glass Impressions.--A perfectly clean coverglass is placed on a plate or potato culture and gently pressed into it; it is then ...
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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1887 edition. Excerpt: ...the reagents are applied by a pipette at one edge of the cover-glass, and withdrawn from beneath the cover by means of a strip of filter paper placed at the opposite edge. Cover-glass Impressions.--A perfectly clean coverglass is placed on a plate or potato culture and gently pressed into it; it is then carefully raised by forceps and allowed to dry after being passed through the flame three times. The growth in this way is bodily transferred to the cover-glass. Gram's Method.--Specimens prepared by this method should be kept in absolute alcohol, from which they are transferred'at once to Weigert's or Ehrlich's, gentian violet, or fuchsine and aniline water solutions, where the)' remain for several minutes, though tubercle sections should be kept at least twenty-four hours, afterwards washed for three minutes in alcohol, and then in a solution of 10 parts of iodine, 20 parts of iodide of potassium, and 3000 parts of water, until a dark blue violet is changed to a dark purple red. Now wash in alcohol until most of the color has disappeared, then clarify in oil of cloves. Such sections are to be mounted at once in balsam, when the tissues will be seen to have a faint yellow color, and the bacteria are deep blue or black. Most micro-organisms can be stained by this method, but some require a different treatment. For example, the bacillus of glanders is best stained with an alkaline solution of methyl blue, which is prepared (Schutz) by making a one-tenth part per thousand watery solution of caustic potash and adding one-third the bulk of a saturated solution of methyl blue. These specimens are then treated with dilute acetic acid, and mounted in the regular way. So also the typhoid bacilli are said to be stained best by this method--not so well by...
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New. Trade paperback (US). Glued binding. 98 p. In Stock. 100% Money Back Guarantee. Brand New, Perfect Condition, allow 4-14 business days for standard shipping. To Alaska, Hawaii, U.S. protectorate, P.O. box, and APO/FPO addresses allow 4-28 business days for Standard shipping. No expedited shipping. All orders placed with expedited shipping will be cancelled. Over 3, 000, 000 happy customers.
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PLEASE NOTE, WE DO NOT SHIP TO DENMARK. New Book. Shipped from UK in 4 to 14 days. Established seller since 2000. Please note we cannot offer an expedited shipping service from the UK.
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PLEASE NOTE, WE DO NOT SHIP TO DENMARK. New Book. Shipped from UK in 4 to 14 days. Established seller since 2000. Please note we cannot offer an expedited shipping service from the UK.
